IGN - If you don't know by now, Regina hates Mary Margaret. Oh wait, Regina hates everyone. Be that as it may, this episode reveals why she especially has it in for Snow White. Since she also has great contempt for love, I assumed the emotion had something to do with it and I was right. Snow White indirectly and innocently caused Regina's true love to be killed.
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So now we know why she has her mind set to ruin Snow White and won't stop at anything or anyone getting in her way.